Rab18 Polyklonaler Antikörper

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• KD/KO Validated

Rab18 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 11304-1-AP

Synonyme

Ras-related protein Rab-18, Ras related protein Rab 18, RAB18LI1

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  WB analysis using 11304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11304-1-AP (Rab18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11304-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11304-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11304-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11304-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with mouse testis tissue lysate 1120 ug.

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  IP experiment of rat testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with rat testis tissue lysate 4000 ug.

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  IHC staining of mouse brain using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11304-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Rab18 antibody (11304-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Hodengewebe, Maushodengewebe, PC-3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 16 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

11304-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA Rab18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rab18 fusion protein Ag1841
• Vollständiger Name: RAB18, member RAS oncogene family
• Berechnetes Molekulargewicht: 206 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC015014
• Gene symbol: RAB18
• Gene ID (NCBI): 22931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.

Publikationen

Species	Application	Title
human	WB,IF	J Clin Invest; RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia; Authors - Nathalie Launay
monkey	WB	J Cell Biol; Rab18 and a Rab18 GEF complex are required for normal ER structure.; Authors - Andreas Gerondopoulos
mouse	IF	Dev Cell; KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice; Authors - Shuo Wang
human	WB	PLoS Pathog; Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets.; Authors - Salloum Shadi S
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
human	WB,IHC	Carcinogenesis; Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.; Authors - You Xiaona X ; KD Validated