RAB1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17824-1-PBS

Synonyme

EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

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  WB analysis using 17824-1-AP (same clone as 17824-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 17824-1-AP (same clone as 17824-1-PBS)

  HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17824-1-AP (same clone as 17824-1-PBS)

  WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells. This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17824-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17824-1-PBS

  A375 cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17824-1-AP (same clone as 17824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 17824-1-AP (same clone as 17824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 17824-1-AP (same clone as 17824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 17824-1-AP (same clone as 17824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 17824-1-AP (same clone as 17824-1-PBS)

  Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17824-1-AP (same clone as 17824-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17824-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17824-1-PBS bindet in WB, IHC, IF/ICC, ELISA RAB1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB1B fusion protein Ag12197
• Vollständiger Name: RAB1B, member RAS oncogene family
• Berechnetes Molekulargewicht: 201 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC071169
• Gene symbol: RAB1B
• Gene ID (NCBI): 81876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport,  was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes  that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).