Rab23 Monoklonaler Antikörper

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• KD/KO Validated

Rab23 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3E12B6

Kat-Nr. : 60056-1-Ig

Synonyme

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Galerie der Validierungsdaten

• 

  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60056-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

60056-1-Ig bindet in WB,ELISA Rab23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rab23 fusion protein Ag1588
• Vollständiger Name: RAB23, member RAS oncogene family
• Berechnetes Molekulargewicht: 237 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC015021
• Gene symbol: RAB23
• Gene ID (NCBI): 51715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publikationen

Species	Application	Title
human,mouse	WB	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated