RAB24 Polyklonaler Antikörper

RAB24 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11445-1-AP

Synonyme

RAB24, Ras related protein Rab 24

---

Galerie der Validierungsdaten

• 

  WB analysis using 11445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11445-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11445-1-AP

  IP result of anti-RAB24(IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human gliomas using 11445-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11445-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, humanes Skelettmuskelgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11445-1-AP bindet in WB, IP, IHC, ELISA RAB24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB24 fusion protein Ag1995
• Vollständiger Name: RAB24, member RAS oncogene family
• Berechnetes Molekulargewicht: 203 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC021263
• Gene symbol: RAB24
• Gene ID (NCBI): 53917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking. Unlike other Rab family members, RAB24 does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB. It Interacts with ZFYVE20. Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.

Publikationen

Species	Application	Title
canine	IHC	PLoS Genet; Canine Hereditary Ataxia in Old English Sheepdogs and Gordon Setters Is Associated with a Defect in the Autophagy Gene Encoding RAB24.; Authors - Caryline Agler
human	WB	Dose Response; Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.; Authors - Jianfa Yu