RAB27A Monoklonaler Antikörper

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• KD/KO Validated

RAB27A Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C4B8

Kat-Nr. : 66058-1-PBS

Synonyme

1C4B8, EC:3.6.5.2, GTP binding protein Ram, GTP-binding protein Ram, Rab 27

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  WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

  WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66058-1-Ig (same clone as 66058-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66058-1-PBS bindet in WB, IHC, Indirect ELISA RAB27A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB27A fusion protein Ag12382
• Vollständiger Name: RAB27A, member RAS oncogene family
• Berechnetes Molekulargewicht: 221 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC107680
• Gene symbol: RAB27A
• Gene ID (NCBI): 5873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.