RAB31 Polyklonaler Antikörper

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RAB31 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15485-1-AP

Synonyme

RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

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  WB analysis using 15485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15485-1-AP

  WB result of RAB31 antibody (15485-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB31 transfected HeLa cells.

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  WB analysis using 15485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15485-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15485-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15485-1-AP

  IP result of anti-RAB31 (IP:15485-1-AP, 4ug; Detection:15485-1-AP 1:1000) with K-562 cells lysate 1640 ug.

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  IHC staining of human liver cancer using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15485-1-AP (RAB31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

15485-1-AP bindet in WB, IHC, IF, IP, ELISA RAB31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB31 fusion protein Ag7833
• Vollständiger Name: RAB31, member RAS oncogene family
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC001148
• Gene symbol: RAB31
• Gene ID (NCBI): 11031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes.

Publikationen

Species	Application	Title
human	WB	J Transl Med; From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses; Authors - Tang Biao
human	WB,IF	J Extracell Biol; RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer; Authors - Yu-Chan Chang