RAB32 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB32 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 10999-1-AP

Synonyme

Ras-related protein Rab-32, Ras related protein Rab 32

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  WB analysis using 10999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 10999-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 10999-1-AP

  IP result of anti-RAB32 (IP:10999-1-AP, 4ug; Detection:10999-1-AP 1:500) with HEK-293T cells lysate 1560 ug.

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  IHC staining of human colon using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 10999-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Kolongewebe
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10999-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RAB32 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB32 fusion protein Ag1450
• Vollständiger Name: RAB32, member RAS oncogene family
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC015061
• Gene symbol: RAB32
• Gene ID (NCBI): 10981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Dis; Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission; Authors - Pin Chen ; KD Validated
mouse	WB,IF	mSphere; Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence; Authors - Chenglong Rao ; KD Validated
human,mouse	WB,CoIP	J Cell Biol; LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis; Authors - Jinglin Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

deng (Verified Customer) (01-31-2025)

The antibody detects human endogenous Rab32.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 dilution for WB, and 1: 250 dilution for IF
• Cell Tissue Type: Mouse brain injected with human AAV-Rab32, human RPE1 cells

RAB32 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution for WB, and 1: 250 dilution for IF dilution) in Mouse brain injected with human AAV-Rab32, human RPE1 cells (Cat no:10999-1-AP)