RAB33A Monoklonaler Antikörper

RAB33A Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C7F11

Kat-Nr. : 68389-1-PBS

Synonyme

RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10

---

• 

  WB analysis using 68389-1-Ig (same clone as 68389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 68389-1-Ig (same clone as 68389-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 68389-1-Ig (same clone as 68389-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68389-1-PBS bindet in WB, IF/ICC, Indirect ELISA RAB33A und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB33A fusion protein Ag33188
• Vollständiger Name: RAB33A, member RAS oncogene family
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009996
• Gene symbol: RAB33A
• Gene ID (NCBI): 9363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).