RAB33B Polyklonaler Antikörper

RAB33B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27349-1-AP

Synonyme

Ras-related protein Rab-33B, Ras related protein Rab 33B

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  WB analysis of HeLa using 27349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27349-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27349-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 27349-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 27349-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, MCF-7-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27349-1-AP bindet in WB, IHC, ELISA RAB33B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB33B fusion protein Ag24181
• Vollständiger Name: RAB33B, member RAS oncogene family
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC036064
• Gene symbol: RAB33B
• Gene ID (NCBI): 83452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.; Authors - Mio Kawabata