RAB33B Polyklonaler Antikörper

RAB33B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27349-1-PBS

Synonyme

Ras-related protein Rab-33B, Ras related protein Rab 33B

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  WB analysis of HeLa using 27349-1-AP (same clone as 27349-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  WB analysis using 27349-1-AP (same clone as 27349-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 27349-1-AP (same clone as 27349-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 27349-1-AP (same clone as 27349-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27349-1-PBS bindet in WB, IHC, Indirect ELISA RAB33B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB33B fusion protein Ag24181
• Vollständiger Name: RAB33B, member RAS oncogene family
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC036064
• Gene symbol: RAB33B
• Gene ID (NCBI): 83452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.