RAB34 Polyklonaler Antikörper

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• KD/KO Validated

RAB34 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 27435-1-AP

Synonyme

Ras-related protein Rab-34, Ras related protein Rah, Ras related protein Rab 39, Ras related protein Rab 34, RAH

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  WB analysis using 27435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27435-1-AP (RAB34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 7 publications below

Produktinformation

27435-1-AP bindet in WB, IHC, IF, ELISA RAB34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB34 fusion protein Ag26696
• Vollständiger Name: RAB34, member RAS oncogene family
• Berechnetes Molekulargewicht: 251 aa, 28 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC016841
• Gene symbol: RAB34
• Gene ID (NCBI): 83871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.

Publikationen

Species	Application	Title
mouse	IF, WB	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga ; KD Validated
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	WB,IF	Biomedicines; Signaling Switching from Hedgehog-GLI to MAPK Signaling Potentially Serves as a Compensatory Mechanism in Melanoma Cell Lines Resistant to GANT-61; Authors - Nikolina Piteša
human	WB	Hum Mol Genet; Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome; Authors - Ange-Line Bruel
human	IF	Elife; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie
human	WB,IF	Elife; Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages; Authors - Tomoharu Kanie