RAB37 Polyklonaler Antikörper

RAB37 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13051-1-AP

Synonyme

RAB37, Ras related protein Rab 37

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Galerie der Validierungsdaten

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13051-1-AP

  IP result of anti-RAB37(IP:13051-1-AP, 4ug; Detection:13051-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen tissue using 13051-1-AP

  IHC result from Barry Bradford of 13051-1-AP (1:1,000) in Mouse Spleen.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

13051-1-AP bindet in WB, IP, IHC, IF, ELISA RAB37 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB37 fusion protein Ag3717
• Vollständiger Name: RAB37, member RAS oncogene family
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016615
• Gene symbol: RAB37
• Gene ID (NCBI): 326624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
mouse	WB,IHC,IF,IP	Autophagy; Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo; Authors - Shan-Ying Wu
human	WB	Cell Death Differ; RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly.; Authors - Yue Sheng
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
canine	WB	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui