RAB37 Rekombinanter Antikörper

RAB37 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240838D6

Kat-Nr. : 83740-4-RR

Synonyme

Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6

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  WB analysis using 83740-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83740-4-RR (RAB37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 83740-4-RR

  IP result of anti-RAB37 (IP:83740-4-RR, 4ug; Detection:83740-4-RR 1:2000) with mouse brain tissue lysate 1840 ug.

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  Affinity and Kinetic Characterization of 83740-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83740-4-RR against Human RAB37 were performed. The affinity constant is 1.25 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83740-4-RR bindet in WB, IP, ELISA RAB37 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAB37 fusion protein Ag3717
• Vollständiger Name: RAB37, member RAS oncogene family
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016615
• Gene symbol: RAB37
• Gene ID (NCBI): 326624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.