RAB38 Polyklonaler Antikörper

RAB38 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12234-1-AP

Synonyme

Melanoma antigen NY MEL 1, RAB 38, Ras related protein Rab 38, Ras-related protein Rab-38

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  WB analysis using 12234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12234-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12234-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12234-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HaCaT-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

12234-1-AP bindet in WB, IHC, IF/ICC, ELISA RAB38 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB38 fusion protein Ag2874
• Vollständiger Name: RAB38, member RAS oncogene family
• Berechnetes Molekulargewicht: 211 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC015808
• Gene symbol: RAB38
• Gene ID (NCBI): 23682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB38 (also known as rrGTPbp or NY-MEL-1) is a member of the Rab small GTPase family, which regulates intracellular vesicle trafficking and membrane transport. RAB38 is specifically expressed in lysosome-related organelle (LRO)-containing tissues and cells, such as melanocytes and platelets, where it interacts with its paralogue RAB32 to regulate melanosome biogenesis and transport melanogenic enzymes (e.g., tyrosinase, TRP1, and TRP2) from early/recycling endosomes to maturing melanosomes.

Publikationen

Species	Application	Title
human	WB	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya
mouse	IF	JBMR Plus; Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets; Authors - Yasunori Omata