RAB39 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB39 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13355-1-AP

Synonyme

Rab 39, RAB39, RAB39A, Ras related protein Rab 39A

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13355-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13355-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13355-1-AP

  IP Result of anti-RAB39 (IP:13355-1-AP, 4ug; Detection:13355-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13355-1-AP bindet in WB, IP, IHC, IF, ELISA RAB39 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB39 fusion protein Ag4151
• Vollständiger Name: RAB39, member RAS oncogene family
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC028064
• Gene symbol: RAB39
• Gene ID (NCBI): 54734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KD Validated
human	WB	Oncotarget; Prominent role of RAB39A-RXRB axis in cancer development and stemness.; Authors - Tokuhiro Chano
human	IF	J Biochem; RAB39A: a Rab small GTPase with a prominent role in cancer stemness.; Authors - Tokuhiro Chano
canine	WB,IP	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui