RAB39B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB39B Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 12162-1-AP

Synonyme

RAB39B, Ras related protein Rab 39B

---

Galerie der Validierungsdaten

• 

  WB analysis of fetal human brain using 12162-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12162-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12162-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12162-1-AP

  IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:1000) with SH-SY5Y cells lysate 2200 ug.

• 

  IP experiment of mouse brain using 12162-1-AP

  IP Result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lymphoma using 12162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12162-1-AP (RAB39B antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe, Maushirngewebe, Y79-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

12162-1-AP bindet in WB, IP, IHC, IF, ELISA RAB39B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB39B fusion protein Ag2803
• Vollständiger Name: RAB39B, member RAS oncogene family
• Berechnetes Molekulargewicht: 213 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC009714
• Gene symbol: RAB39B
• Gene ID (NCBI): 116442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KO Validated
mouse	WB	Am J Hum Genet; Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology.; Authors - Gabrielle R Wilson
human	IP	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
human	WB	Mov Disord; Clinical and Neuropathological Features Associated With Loss of RAB39B.; Authors - Yujing Gao
mouse	IF,WB	Acta Neuropathol Commun; Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels.; Authors - Shangxi Xiao
human	WB,IF	Brain Pathol; RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies.; Authors - David J Koss

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

David (Verified Customer) (09-25-2019)

The antibodiy works well in membrane enriched fractionates generated from human brain homogenate. Note that several higher molecular weight bands are seen when using crude lysates generated from the same samples.Image details: lane 1 = molecular weight ladder, lanes 2-7 = membrane enriched fractionates (2µg/lane).Membrane blocked in 5% milk powder containing TBST overnight at 4oC. Primary antibody: RAB39B (1:1000) in 5% BSA containing TBST, 1hr incubation at RT. Secondar antibody: y Goat anti Rabbit-HRP (1:5000, 1hr RT) in 5% milk powder containing TBST.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue

RAB39B Antibody Western Blot, validation (1:1000 dilution) in Human brain tissue (Cat no:12162-1-AP)