RAB3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB3A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15029-1-AP

Synonyme

RAB3A, Ras related protein Rab 3A

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Galerie der Validierungsdaten

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15029-1-AP

  IP result of anti-RAB3A(IP:15029-1-AP, 4ug; Detection:15029-1-AP 1:1000) with mouse brain tissue lysate 1920 ug.

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

15029-1-AP bindet in WB, IP, IHC, IF, ELISA RAB3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3A fusion protein Ag1903
• Vollständiger Name: RAB3A, member RAS oncogene family
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC011782
• Gene symbol: RAB3A
• Gene ID (NCBI): 5864
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3A is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Mol Oncol; Overexpression of synaptic vesicle protein Rab GTPase 3C promotes vesicular exocytosis and drug resistance in colorectal cancer cells; Authors - Yu-Chan Chang
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
human	WB,IHC	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu ; KD Validated
mouse	WB	FASEB J; Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain.; Authors - Lakshmana Madepalli K MK
mouse	WB	PLoS One; RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain.; Authors - Hongjie Wang
human	WB	Biochem Pharmacol; Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7.; Authors - Shuang Liu