RAB3B/C Polyklonaler Antikörper

RAB3B/C Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15774-1-AP

Synonyme

RAB3B, Ras related protein Rab 3B, Ras-related protein Rab-3B

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  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 15774-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15774-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15774-1-AP

  Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 15774-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAB3B/C Polyclonal antibody (15774-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15774-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA RAB3B/C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3B/C fusion protein Ag8441
• Vollständiger Name: RAB3B, member RAS oncogene family
• Berechnetes Molekulargewicht: 219 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC005035
• Gene symbol: RAB3B
• Gene ID (NCBI): 5865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Ann Neurol; PMID: 32167609; Authors - Jimena Monzón-Sandoval
mouse	WB	Hum Mol Genet; Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.; Authors - Gaynor A Smith
human	WB,IF	Cell Death Dis; Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B; Authors - Jinxiu Hu
human	WB	Adv Sci (Weinh); Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase; Authors - Johannes Naß
human	IHC	Sci Rep; Pan-cancer analysis reveals correlation between RAB3B expression and tumor heterogeneity, immune microenvironment, and prognosis in multiple cancers; Authors - Xu-Sheng Liu