RAB3C Polyklonaler Antikörper

RAB3C Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10788-1-AP

Synonyme

RAB3C, Ras related protein Rab 3C

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Galerie der Validierungsdaten

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  WB analysis using 10788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10788-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10788-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10788-1-AP

  IP Result of anti-RAB3C (IP:10788-1-AP, 4ug; Detection:10788-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 10788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Mauslungengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10788-1-AP bindet in WB, IP, IHC, IF, ELISA RAB3C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3C fusion protein Ag1241
• Vollständiger Name: RAB3C, member RAS oncogene family
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC013033
• Gene symbol: RAB3C
• Gene ID (NCBI): 115827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

Publikationen

Species	Application	Title
mouse	WB,IF	Proc Natl Acad Sci U S A; The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells.; Authors - Zou Liyun L
mouse	IF	PLoS Genet; Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.; Authors - Maha El Shahawy
human	WB	Front Endocrinol (Lausanne); Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension; Authors - He Ma
human	IHC	Signal Transduct Target Ther; Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers.; Authors - Wanxue Xu
human	IHC	Signal Transduct Target Ther; Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers; Authors - Wanxue Xu