RAB3C Polyklonaler Antikörper

RAB3C Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10788-1-PBS

Synonyme

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  WB analysis using 10788-1-AP (same clone as 10788-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 10788-1-AP (same clone as 10788-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10788-1-AP (same clone as 10788-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  IP result of anti-RAB3C (IP:10788-1-AP, 4ug; Detection:10788-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10788-1-PBS bindet in WB, IHC, IP, Indirect ELISA RAB3C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3C fusion protein Ag1241
• Vollständiger Name: RAB3C, member RAS oncogene family
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC013033
• Gene symbol: RAB3C
• Gene ID (NCBI): 115827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.