RAB3GAP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB3GAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4A3A1

Rezensionen (1)

Kat-Nr. : 66688-1-Ig

Synonyme

4A3A1, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130

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  WB analysis using 66688-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66688-1-Ig

  WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells.

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  IHC staining of mouse brain using 66688-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66688-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66688-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66688-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66688-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66688-1-Ig bindet in WB, IHC, IF/ICC, ELISA RAB3GAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB3GAP1 fusion protein Ag17710
• Vollständiger Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Berechnetes Molekulargewicht: 981 aa, 111 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC146809
• Gene symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

bing (Verified Customer) (08-04-2021)

very good Ab to probe RAB3GAP1 by WB with the right MW of 130 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain and postmortembrain lysates

RAB3GAP1 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:66688-1-Ig)