RAB43 Monoklonaler Antikörper

RAB43 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3B2A8

Rezensionen (3)

Kat-Nr. : 66654-1-Ig

Synonyme

ISY1, RAB11B, RAB41, Ras related protein Rab 41, Ras related protein Rab 43

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• 

  WB analysis of human brain using 66654-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66654-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66654-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66654-1-Ig bindet in WB, ELISA RAB43 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB43 fusion protein Ag26911
• Vollständiger Name: RAB43, member RAS oncogene family
• Berechnetes Molekulargewicht: 212 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC062319
• Gene symbol: RAB43
• Gene ID (NCBI): 339122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ras-related GTP-binding protein 43 (RAB43) is a member of the Ras superfamily. RAB43 is mainly located in endoplasmic reticulum and Golgi, and plays a role as a key regulator of vesicle movement, signal transduction and tethering membrane events in membrane trafficking.

Publikationen

Species	Application	Title
mouse,human	WB	J Cell Sci; ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane; Authors - Juan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibody recognise a specific band of human Rab43. Overnight incubation at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

RAB43 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:66654-1-Ig)

FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody for Rab43 detection

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

RAB43 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:66654-1-Ig)

FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB. HEK293T Wt lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RAB43 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:66654-1-Ig)