RAB5A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB5A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Rezensionen (4)

Kat-Nr. : 11947-1-AP

Synonyme

RAB5, RAB5A, Ras related protein Rab 5A

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Galerie der Validierungsdaten

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  WB analysis using 11947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11947-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11947-1-AP

  IP result of anti-RAB5A(IP:11947-1-AP, 4ug; Detection:11947-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of human gliomas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11947-1-AP (RAB5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Pankreasgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 2 publications below

Produktinformation

11947-1-AP bindet in WB, IP, IHC, IF, ELISA RAB5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB5A fusion protein Ag2549
• Vollständiger Name: RAB5A, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001267
• Gene symbol: RAB5A
• Gene ID (NCBI): 5868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB,IP	Autophagy; Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.; Authors - Amy H Ponsford
mouse	WB	Autophagy; RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.; Authors - Xin Jin
mouse	WB,IHC	Diabetes; Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.; Authors - Linyan Shen ; KD Validated
rat	WB, IF	Sci Signal; Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.; Authors - Kong-Yan Wu
human	WB,IF,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sophy (Verified Customer) (02-27-2023)

The antibody was used at 1:1000 for western blot. The signal showed up but not as strong as expected. But since this was the only antibody purchased for Rab5A, I had no comparison to antibodies to other vendors.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells

FH

Xin (Verified Customer) (01-24-2022)

Very good antibody in WB (25 kD) with a high titer

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse brain lysate

FH

Tom (Verified Customer) (11-13-2020)

HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RAB5A Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11947-1-AP)

FH

Aamir (Verified Customer) (01-19-2020)

Used in HEK cells. Good signal for WB and IF

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: HEKs

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