RAB5A Monoklonaler Antikörper

RAB5A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6A5

Kat-Nr. : 66339-1-PBS

Synonyme

RAB5, 1B6A5, EC:3.6.5.2, Ras related protein Rab 5A, Ras-related protein Rab-5A

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  WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66339-1-Ig (same clone as 66339-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66339-1-Ig (same clone as 66339-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66339-1-Ig(RAB5A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66339-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RAB5A und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB5A fusion protein Ag16781
• Vollständiger Name: RAB5A, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001267
• Gene symbol: RAB5A
• Gene ID (NCBI): 5868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.