RAB5A-Specific Polyklonaler Antikörper

RAB5A-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20228-1-AP

Synonyme

RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

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Galerie der Validierungsdaten

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  WB analysis of human brain using 20228-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20228-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20228-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20228-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20228-1-AP

  IP Result of anti-RAB5A-Specific (IP:20228-1-AP, 4ug; Detection:20228-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maus-Kolongewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20228-1-AP bindet in WB, IP, IHC, ELISA RAB5A-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB5A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_004162
• Gene symbol: RAB5A
• Gene ID (NCBI): 5868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.