RAB7A Rekombinanter Antikörper

RAB7A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241948B4

Kat-Nr. : 84741-1-PBS

Synonyme

RAB7, 241948B4, EC:3.6.5.2, Ras related protein Rab 7a, Ras-related protein Rab-7a

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  WB analysis using 84741-1-RR (same clone as 84741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 84741-1-RR (same clone as 84741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84741-1-RR (same clone as 84741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84741-1-RR (same clone as 84741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84741-1-RR (same clone as 84741-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 84741-1-RR (same clone as 84741-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84741-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RAB7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB7A, member RAS oncogene family
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_004637
• Gene symbol: RAB7A
• Gene ID (NCBI): 7879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.