RAB8A Polyklonaler Antikörper

RAB8A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30636-1-AP

Synonyme

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

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  WB analysis using 30636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30636-1-AP (RAB8A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 30636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 30636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30636-1-AP bindet in WB, IF/ICC, ELISA RAB8A und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB8A fusion protein Ag30567
• Vollständiger Name: RAB8A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_005370
• Gene symbol: RAB8A
• Gene ID (NCBI): 4218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.