• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAB8A Rekombinanter Antikörper

RAB8A Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251429D1

Kat-Nr. : 86540-5-PBS

Synonyme

EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel, RAB8

Filter:
  • Western Blot (WB) analysis of various lysates using RAB8A Recombinant monoclonal antibody (86540-5-RR)

    WB analysis using 86540-5-RR (same clone as 86540-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86540-5-RR (RAB8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86540-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86540-5-RR

    Affinity and Kinetic Characterization of 86540-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86540-5-RR against Human RAB8A were performed. The affinity constant is 4.84 nM.

Geprüfte Anwendungen

Produktinformation

86540-5-PBS bindet in WB, Indirect ELISA RAB8A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAB8A fusion protein Ag30567
Vollständiger Name RAB8A, member RAS oncogene family
Berechnetes Molekulargewicht 24 kDa
Beobachtetes Molekulargewicht 24 kDa
GenBank-ZugangsnummerNM_005370
Gene symbol RAB8A
Gene ID (NCBI) 4218
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.