RABEPK/p40 Polyklonaler Antikörper

RABEPK/p40 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15105-1-AP

Synonyme

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

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  WB analysis of HepG2 using 15105-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15105-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15105-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15105-1-AP

  IP result of anti-RABEPK/p40 (IP:15105-1-AP, 4ug; Detection:15105-1-AP 1:1000) with HeLa cells lysate 2080 ug.

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  IHC staining of human lung squamous cell carcinoma using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15105-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEPK/p40 antibody (15105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human lung squamous cell carcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

15105-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RABEPK/p40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABEPK/p40 fusion protein Ag2593
• Vollständiger Name: Rab9 effector protein with kelch motifs
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC065725
• Gene symbol: RABEPK/p40
• Gene ID (NCBI): 10244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Publikationen

Species	Application	Title
human	IF	Adv Sci (Weinh); In Situ Transformable Nanoparticle Effectively Suppresses Bladder Cancer by Damaging Mitochondria and Blocking Mitochondrial Autophagy Flux; Authors - Yulin Lv
mouse	WB	Redox Biol; Coronin 1a-mediated F-actin disassembly controls effector function in murine neutrophils; Authors - Anton Shaverskyi