RABEPK/p40 Monoklonaler Antikörper

RABEPK/p40 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E11A3

Kat-Nr. : 66622-1-PBS

Synonyme

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

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  WB analysis of Jurkat using 66622-1-Ig (same clone as 66622-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66622-1-Ig (same clone as 66622-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66622-1-Ig (same clone as 66622-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66622-1-Ig (same clone as 66622-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66622-1-Ig (same clone as 66622-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66622-1-Ig (same clone as 66622-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66622-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RABEPK/p40 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RABEPK/p40 fusion protein Ag7796
• Vollständiger Name: Rab9 effector protein with kelch motifs
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC065725
• Gene symbol: RABEPK/p40
• Gene ID (NCBI): 10244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.