RABGAP1L Rekombinanter Antikörper

RABGAP1L Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240663G12

Kat-Nr. : 83698-4-PBS

Synonyme

Rab GTPase-activating protein 1-like, isoform 10, 240663G12

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  WB analysis using 83698-4-RR (same clone as 83698-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83698-4-RR (RABGAP1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83698-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83698-4-RR (same clone as 83698-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RABGAP1L Recombinant antibody (83698-4-RR, Clone:240663G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83698-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83698-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83698-4-RR against Human RABGAP1L were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83698-4-PBS bindet in WB, FC (Intra), ELISA RABGAP1L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RABGAP1L fusion protein Ag4856
• Vollständiger Name: RAB GTPase activating protein 1-like
• Berechnetes Molekulargewicht: 815 aa, 93 kDa
• Beobachtetes Molekulargewicht: 100 kDa,140 kDa
• GenBank-Zugangsnummer: BC041888
• Gene symbol: RABGAP1L
• Gene ID (NCBI): 9910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.