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RABIF Polyklonaler Antikörper

RABIF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29113-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using RABIF Polyclonal antibody (29113-1-AP)

    WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of human breast cancer using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RABIF Polyclonal antibody (29113-1-AP)

    IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29113-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RABIF und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RABIF fusion protein Ag30761
Vollständiger Name RAB interacting factor
Berechnetes Molekulargewicht 123 aa, 14 kDa
Beobachtetes Molekulargewicht14 kDa
GenBank-ZugangsnummerBC018488
Gene symbol RABIF
Gene ID (NCBI) 5877
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).