RABL2B Polyklonaler Antikörper

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• KD/KO Validated

RABL2B Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11588-1-AP

Synonyme

Rab like protein 2B, Rab-like protein 2B

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  WB analysis using 11588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11588-1-AP

  WB result of RABL2B antibody (11588-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RABL2B transfected HepG2 cells.

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  WB analysis of human brain using 11588-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11588-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11588-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11588-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of fetal human brain using 11588-1-AP

  IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug.

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  IHC staining of mouse kidney using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HepG2-Zellen, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, Maushirngewebe
• Erfolgreiche IP: fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

11588-1-AP bindet in WB, IHC, IF, IP, ELISA RABL2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABL2B fusion protein Ag2189
• Vollständiger Name: RAB, member of RAS oncogene family-like 2B
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC024281
• Gene symbol: RABL2B
• Gene ID (NCBI): 11158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; RABL2 positively controls localization of GPCRs in mammalian primary cilia.; Authors - Izumi Dateyama
human	WB	bioRxiv; Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling; Authors - Geralle Powell-Rodgers