RABL2B Polyklonaler Antikörper

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• KD/KO Validated

RABL2B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11588-1-PBS

Synonyme

Rab like protein 2B, Rab-like protein 2B

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  WB analysis using 11588-1-AP (same clone as 11588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11588-1-AP (same clone as 11588-1-PBS)

  WB result of RABL2B antibody (11588-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RABL2B transfected HepG2 cells. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11588-1-AP (same clone as 11588-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11588-1-AP (same clone as 11588-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 11588-1-AP (same clone as 11588-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 11588-1-AP (same clone as 11588-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  IP experiment of fetal human brain using 11588-1-AP (same clone as 11588-1-PBS)

  IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11588-1-AP (same clone as 11588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11588-1-AP (same clone as 11588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11588-1-AP (same clone as 11588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11588-1-AP (same clone as 11588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11588-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11588-1-PBS bindet in WB, IHC, IP, Indirect ELISA RABL2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABL2B fusion protein Ag2189
• Vollständiger Name: RAB, member of RAS oncogene family-like 2B
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC024281
• Gene symbol: RABL2B
• Gene ID (NCBI): 11158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).