RABL3 Polyklonaler Antikörper

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• KD/KO Validated

RABL3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11916-1-PBS

Synonyme

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  WB analysis of human lung using 11916-1-AP (same clone as 11916-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11916-1-AP (same clone as 11916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11916-1-AP (same clone as 11916-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 11916-1-AP (same clone as 11916-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 11916-1-PBS

  HUVEC cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 11916-1-AP (same clone as 11916-1-PBS)

  IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 11916-1-AP (same clone as 11916-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11916-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA RABL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABL3 fusion protein Ag2531
• Vollständiger Name: RAB, member of RAS oncogene family-like 3
• Berechnetes Molekulargewicht: 236 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC020832
• Gene symbol: RABL3
• Gene ID (NCBI): 285282
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).