Rac1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Rac1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(112)

Kat-Nr. : 24072-1-AP

Synonyme

MIG5, Cell migration-inducing gene 5 protein, EC:3.6.5.2, p21 Rac1, p21-Rac1

---

• 

  WB analysis using 24072-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24072-1-AP (Rac1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24072-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 24072-1-AP (Rac1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:500
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 102 publications below
• IHC: See 12 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Produktinformation

24072-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Rac1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rac1 fusion protein Ag17779
• Vollständiger Name: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC004247
• Gene symbol: RAC1
• Gene ID (NCBI): 5879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Thymic egress and peripheral T cell homeostasis regulated by Rho GTPase-activating protein 30; Authors - Huiling Zhang
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
rat	WB,coIP	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
mouse,human	WB,IHC	Cancer Res; CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder.; Authors - Xin Chen
		Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang