RAC1 Monoklonaler Antikörper

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• KD/KO Validated

RAC1 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

4A4B11

Kat-Nr. : CL488-66122

Synonyme

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

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  IF Staining of HeLa using CL488-66122

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated RAC1 antibody (CL488-66122, Clone: 4A4B11 ) at dilution of 1:100.

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  IF Staining of HeLa using CL488-66122

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66122 (RAC1 antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-66122 bindet in IF/ICC RAC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAC1 fusion protein Ag17779
• Vollständiger Name: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC004247
• Gene symbol: RAC1
• Gene ID (NCBI): 5879
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Publikationen

Species	Application	Title
human	IF	Cell Death Dis; MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling.; Authors - Huiyi Tang
		Stem Cell Res Ther; Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regeneration; Authors - Zijie Wang