RAC2 Polyklonaler Antikörper

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• KD/KO Validated

RAC2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20573-1-AP

Synonyme

Small G protein, Ras-related C3 botulinum toxin substrate 2, p21-Rac2, p21 Rac2, HSPC022

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  WB analysis of Jurkat using 20573-1-AP

  WB result of RAC2 antibody (20573-1-AP, 1:600) with si-control and si-RAC2 transfected Jurkat cells..

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  WB analysis of Jurkat using 20573-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 20573-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of MOLT-4 using 20573-1-AP

  IP result of anti-RAC2 (IP:20573-1-AP, 4ug; Detection:20573-1-AP 1:500) with MOLT-4 cells lysate 1800 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen
• Erfolgreiche IP: MOLT-4-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20573-1-AP bindet in WB, IP, ELISA RAC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: NM_002872
• Gene symbol: RAC2
• Gene ID (NCBI): 5880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. The antibody is specific to RAC2.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; NEDD4L Suppresses Proliferation and Promotes Apoptosis by Ubiquitinating RAC2 Expression and Acts as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma; Authors - Manlong Qi