RAC1/2/3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAC1/2/3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16117-1-AP

Synonyme

p21 Rac3, RAC1/2/3, RAC3

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Galerie der Validierungsdaten

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  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16117-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16117-1-AP

  IP Result of anti-RAC1/2/3 (IP:16117-1-AP, 3ug; Detection:16117-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below

Produktinformation

16117-1-AP bindet in WB, IP, ELISA RAC1/2/3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAC1/2/3 fusion protein Ag9045
• Vollständiger Name: ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
• Berechnetes Molekulargewicht: 192 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC015197
• Gene symbol: RAC3
• Gene ID (NCBI): 5881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.; Authors - Su Dong
mouse	WB	Front Cell Neurosci; A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.; Authors - Sira A Franchi ; KO Validated
human	WB	Mol Biol Cell; Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.; Authors - Brian J Rosenberg ; KD Validated
human	WB	BMC Cancer; Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.; Authors - Gest Caroline C
mouse	WB	Glycoconj J; Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.; Authors - Erika Di Biase
human	WB	Asian Pac J Cancer Prev; Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.; Authors - Tie-Qin Liu ; KD Validated