RACGAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RACGAP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 13739-1-AP

Synonyme

CYK4, GAP, Gap1, HsCYK 4, HsCYK4

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  WB analysis of K-562 using 13739-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13739-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13739-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13739-1-AP

  IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug; Detection:13739-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IP experiment of HeLa cells using 13739-1-AP

  IP result of anti-RACGAP1 (13739-1-AP for IP and Detection).

• 

  IF Staining of HepG2 using 13739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RACGAP1 antibody (13739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Maushodengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Maushodengewebe, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 2 publications below

Produktinformation

13739-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RACGAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RACGAP1 fusion protein Ag4676
• Vollständiger Name: Rac GTPase activating protein 1
• Berechnetes Molekulargewicht: 632 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC032754
• Gene symbol: RACGAP1
• Gene ID (NCBI): 29127
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Publikationen

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.; Authors - Shixin Liao
mouse	WB	Biochim Biophys Acta Mol Cell Res; MARCH5 ameliorates aortic valve calcification via RACGAP1-DRP1 pathway associated mitochondrial quality control; Authors - Jialiang Zhang
mouse	WB,IHC	Front Pharmacol; Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.; Authors - Fujing Wang
mouse	IF	Mol Biol Cell; Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis.; Authors - Carissa M Heath
human	WB,IHC	Cancers (Basel); Lambda-Carrageenan Enhances the Effects of Radiation Therapy in Cancer Treatment by Suppressing Cancer Cell Invasion and Metastasis through Racgap1 Inhibition.; Authors - Ping-Hsiu Wu
human	WB	Cell Death Dis; Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence.; Authors - Meng-Yao Wang