RACK1; GNB2L1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RACK1; GNB2L1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 27592-1-AP

Synonyme

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

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  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

27592-1-AP bindet in WB, IHC, IF, IP, CoIP, RIP, ELISA RACK1; GNB2L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC019093
• Gene symbol: RACK1
• Gene ID (NCBI): 10399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publikationen

Species	Application	Title
human	WB,CoIP	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang
human,mouse	WB	Cells; Modification of RNF183 via m6A Methylation Mediates Podocyte Dysfunction in Diabetic Nephropathy by Regulating PKM2 Ubiquitination and Degradation; Authors - Dongwei Guo
human	WB,IP,IF	Mol Med; LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation; Authors - Litao Liang
human	WB,IF,IP	J Virol; The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association; Authors - Jingjing Cao
	WB	Cancers (Basel); RACK1 Promotes Meningioma Progression by Activation of NF-κB Pathway via Preventing CSNK2B from Ubiquitination Degradation; Authors - Ali Abdi Maalim
monkey	WB	Virology; RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation; Authors - Xinxian Wang ; KD Validated