RACK1; GNB2L1 Monoklonaler Antikörper

RACK1; GNB2L1 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1E11A7

Kat-Nr. : CL594-66940

Synonyme

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

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  IF Staining of HeLa using CL594-66940

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 RACK1; GNB2L1 antibody (CL594-66940, Clone: 1E11A7 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-66940 bindet in IF/ICC RACK1; GNB2L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC019093
• Gene symbol: RACK1
• Gene ID (NCBI): 10399
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publikationen

Species	Application	Title
mouse	IF	Vet Res; RACK1 mediates NLRP3 inflammasome activation during Pasteurella multocida infection; Authors - Jinrong Ran