RAD17 Polyklonaler Antikörper

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RAD17 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13358-1-PBS

Synonyme

RF-C/activator 1 homolog, R24L, HRAD17

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  WB analysis using 13358-1-AP (same clone as 13358-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13358-1-AP (RAD17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13358-1-AP (same clone as 13358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13358-1-AP (same clone as 13358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13358-1-AP (same clone as 13358-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13358-1-AP (RAD17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13358-1-AP (same clone as 13358-1-PBS)

  Immunofluorescent analysis of Hela cells, using RAD17 antibody 13358-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 13358-1-AP (same clone as 13358-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RAD17 Polyclonal antibody (13358-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13358-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RAD17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD17 fusion protein Ag4171
• Vollständiger Name: RAD17 homolog (S. pombe)
• Berechnetes Molekulargewicht: 681 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC032304
• Gene symbol: RAD17
• Gene ID (NCBI): 5884
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.