RAD18 Polyklonaler Antikörper

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• KD/KO Validated

RAD18 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 18333-1-AP

Synonyme

hHR18, hRAD18, RAD18, RAD18 homolog (S. cerevisiae), RING finger protein 73, RNF73

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 18333-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18333-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18333-1-AP

  IP Result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

18333-1-AP bindet in WB, IP, IHC, IF, ELISA RAD18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD18 fusion protein Ag12944
• Vollständiger Name: RAD18 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 85 kDa
• GenBank-Zugangsnummer: BC001302
• Gene symbol: RAD18
• Gene ID (NCBI): 56852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively. Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Regulation of the DNA damage response on male meiotic sex chromosomes.; Authors - Lu Lin-Yu LY
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	IF	Nucleic Acids Res; REV1 and polymerase ζ facilitate homologous recombination repair.; Authors - Sharma Shilpy S
human	WB	J Cell Sci; RNF168 forms a functional complex with RAD6 during the DNA damage response.; Authors - Liu Chao C
human	IF	Cell Cycle; A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci.; Authors - Corey M Helchowski
human	WB,IF	Mol Pharmacol; DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents.; Authors - Sharma Shilpy S