RAD18 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAD18 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18333-1-PBS

Synonyme

E3 ubiquitin-protein ligase RAD18, EC:2.3.2.27, hHR18, hRAD18, RING finger protein 73

---

• 

  WB analysis of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18333-1-AP (same clone as 18333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 18333-1-AP (same clone as 18333-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18333-1-AP (same clone as 18333-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 18333-1-AP (same clone as 18333-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

  IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

18333-1-PBS bindet in WB, IHC, IP, Indirect ELISA RAD18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD18 fusion protein Ag12944
• Vollständiger Name: RAD18 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 85 kDa
• GenBank-Zugangsnummer: BC001302
• Gene symbol: RAD18
• Gene ID (NCBI): 56852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.