RAD23A Polyklonaler Antikörper

RAD23A Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51033-1-PBS

Synonyme

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  WB analysis of mouse spleen using 51033-1-AP (same clone as 51033-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 51033-1-AP (same clone as 51033-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 51033-1-AP (same clone as 51033-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 51033-1-AP (same clone as 51033-1-PBS)

  IP result of anti-RAD23A (IP:51033-1-AP, 4ug; Detection:51033-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 51033-1-AP (same clone as 51033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 51033-1-AP (RAD23A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

51033-1-PBS bindet in WB, IHC, IP, Indirect ELISA RAD23A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD23A fusion protein Ag0716
• Vollständiger Name: RAD23 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC014026
• Gene symbol: RAD23A
• Gene ID (NCBI): 5886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome. This antibody is a rabbit polyclonal antibody raised against a fusion protein of full length human RAD23A.