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RAD23A Rekombinanter Antikörper

RAD23A Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250716C7

Kat-Nr. : 86132-1-PBS

Synonyme

HHR23A, HR23A

Filter:
  • Western Blot (WB) analysis of Jurkat cells using RAD23A Recombinant monoclonal antibody (86132-1-RR)

    WB analysis of Jurkat using 86132-1-RR (same clone as 86132-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 86132-1-RR (RAD23A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86132-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86132-1-RR

    Affinity and Kinetic Characterization of 86132-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86132-1-RR against Human RAD23A were performed. The affinity constant is 2.99 nM.

Geprüfte Anwendungen

Produktinformation

86132-1-PBS bindet in WB, Indirect ELISA RAD23A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAD23A fusion protein Ag0716
Vollständiger Name RAD23 homolog A (S. cerevisiae)
Berechnetes Molekulargewicht 40 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC014026
Gene symbol RAD23A
Gene ID (NCBI) 5886
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome.