RAD23B Polyklonaler Antikörper

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RAD23B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 12121-1-AP

Synonyme

HHR23B, HR23B, p58, XP-C repair-complementing complex 58 kDa protein

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  WB analysis of HeLa using 12121-1-AP

  WB result of RAD23B antibody (12121-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD23B transfected HeLa cells.

• 

  WB analysis using 12121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12121-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12121-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12121-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12121-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12121-1-AP bindet in WB, IHC, IF, ELISA RAD23B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD23B fusion protein Ag2763
• Vollständiger Name: RAD23 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 409 aa, 43 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC020973
• Gene symbol: RAD23B
• Gene ID (NCBI): 5887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B displays aberrant mobility in SDS-polyacrylamide gels, as it is typically detected as a 58- to 60-kDa protein despite a predicted size of 42 kDa (PMID: 8692695, 12052895).

Publikationen

Species	Application	Title
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human	WB,IHC	Int J Biol Sci; Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.; Authors - Jianjiang Wang
human	WB	Front Oncol; PMID: 31249811; Authors - Ma-Sha Huang
rat	WB	PLoS One; Proteins with Intrinsically Disordered Domains Are Preferentially Recruited to Polyglutamine Aggregates.; Authors - Maggie P Wear
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan