RAD23B Monoklonaler Antikörper

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• KD/KO Validated

RAD23B Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6G9

Kat-Nr. : 67988-1-PBS

Synonyme

2E6G9, HHR23B, HR23B, p58, XP-C repair-complementing complex 58 kDa protein

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  WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67988-1-Ig (same clone as 67988-1-PBS)

  WB result of RAD23B antibody (67988-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD23B transfected HeLa cells. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 67988-1-Ig (same clone as 67988-1-PBS)

  IP result of anti-RAD23B (IP:67988-1-Ig, 4ug; Detection:67988-1-Ig 1:4000) with mouse testis tissue lysate 1560 ug. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67988-1-Ig (same clone as 67988-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67988-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RAD23B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAD23B fusion protein Ag28658
• Vollständiger Name: RAD23 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 409 aa, 43 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC020973
• Gene symbol: RAD23B
• Gene ID (NCBI): 5887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).