RAD50 Polyklonaler Antikörper

RAD50 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (2)

Kat-Nr. : 29390-1-AP

Synonyme

hRad50, RAD50 homolog (S. cerevisiae)

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  WB analysis using 29390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 29390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 29390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of U2OS using 29390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

29390-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA RAD50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD50 fusion protein Ag29949
• Vollständiger Name: RAD50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 154 kDa
• GenBank-Zugangsnummer: NM_005732
• Gene symbol: RAD50
• Gene ID (NCBI): 10111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

Publikationen

Species	Application	Title
human	WB	J Transl Med; Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis; Authors - Zhengyan Chang
mouse	WB	Ecotoxicol Environ Saf; Adolescent cetylpyridinium chloride exposure impairs homologous recombination repair and induces granulosa cell apoptosis and follicular atresia via FOXM1/CREBBP complex suppression; Authors - LiYao Ma
human	IF	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang
human	WB	CNS Neurosci Ther; Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma; Authors - Menglin Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Arnaud (Verified Customer) (09-26-2025)

Work very well at 1:2000 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for WB
• Cell Tissue Type: HeLa

FH

Veronica (Verified Customer) (11-14-2022)

The antibody seems to work correctly. However, further tests need to be done.

• Applications: Immunofluorescence
• Cell Tissue Type: hTERT RPE-1